|
KMID : 0545120040140040829
|
|
Journal of Microbiology and Biotechnology
2004 Volume.14 No. 4 p.829 ~ p.835
|
|
|
Purification and Characterization of the Fibrinolytic Enzyme Produced by Bacillus subtilis KCK-7 from Chungkookjang
|
|
Paik HD
Lee SK/Heo S/Kim SY/Lee HH/Kwon TJ
|
|
|
Abstract
|
|
|
|
A fibrinolytic enzyme has been found in several bacteria isolated from fermented food. This study was carried out to investigate the purification and characteristics of the fibrinolytic enzyme produced by Bacillus subtilis KCK-7 originated from Chungkookjang. The fibrinolytic enzyme was purified to homogeneity from the culture supernatant using ammonium sulfate fractionation and chromatographies on DEAE-cellulose and on Sephadex G-100. The final specific activity of the purified enzyme increased 11.0-fold and the protein amount in the purified enzyme was about 16% of that in the culture supernatant. The molecular weight of the purified enzyme was estimated to be about 45000 by SDSPAGE. The optimum pH and temperature for the enzyme activity were pH 7.0 and 60oC respectively. The enzyme activity was relatively stable up to 60oC over the pH range of 7.0- 10.0. The fibrinolytic enzyme activity increased by Ca2+ and Cu2+ whereas it was inhibited by Hg2+ and Ba2+. In addition it was severely inhibited by PMSF and DFT. It is suggested that the purified enzyme was a serine protease for the fibrinolysis. The purified enzyme could completely hydrolyze fibrin in vitro within 8 h. Hence it is suggested that the purified enzyme can be put into practice as an effective thrombolytic agent.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|